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. Author manuscript; available in PMC: 2015 Dec 14.
Published in final edited form as: J Mater Chem B. 2014 Dec 14;2(46):8154–8164. doi: 10.1039/C4TB00352G

Figure 3. Endosomal escape and pH-dependent membrane disruption of fresh polyplexes.

Figure 3

(A) pH-dependent red blood cell hemolysis assay. All polyplexes investigated exhibited pH-dependent membrane disruption, displaying minimal hemolysis at physiologic pH and switch-like transition into a membrane disruptive confirmation at endo-lysosomal pHs. Data are plotted as mean ± standard error. (B) Confocal microscopy images of MDA-MB-231 cells transfected with fresh 40L or PEI polyplexes with N/P ratio of 10 showing distribution of plasmid (green), endosomes (red), and nuclei (blue). (C) Percentage of plasmid colocalized with Lysotracker dye. Data are plotted as mean ± standard error, and the asterisk indicates statistically significant differences (p < 0.05).