Figure 4. Effect of lyophilization on the size of polymer-pDNA polyplexes measured by DLS.
Polyplexes were diluted to a concentration of 1.5 μg/ml pDNA in DPBS. Hydrodynamic diameter of polyplexes with N/P ratio of 10 before and after lyophilization and subsequent reconstitution. Asterisks indicate significant difference (p < 0.05). Data are plotted as means ± standard deviation.