Figure 5. ζ-potential of fresh polymer-pDNA complexes measured by DLS.
ζ-potential was measured in 1 mM KCl pH 7.0. ζ-potential of 40L and PEI polyplexes increased with N/P ratio. ζ-potential of 40L-pDNA polyplexes approached ∼0 mV for N/P of 5 or greater while ζ-potential of PEI-pDNA polyplexes approached ∼20 mV for N/P of 20 or greater. Data are plotted as mean ± standard deviation.