Table 3.
qPCR and qRT-PCR quantification of nifH and amoA genes representing nitrogen fixation and ammonia oxidation potential and activity (gene abundance and expression).
| Gene Quantification (log copies g dry soil−1)1 |
|||||
|---|---|---|---|---|---|
| Time (Months) | |||||
| Gene | Treatment2 | 0 | 3 | 6 | 9 |
| nifH Abundance | TC | * | * | * | * |
| BG | * | * | * | 7.4 ± 1.0 | |
| QB | * | * | * | * | |
| nifH Expression | TC | ND | ND | ND | ND |
| BG | ND | ND | ND | ND | |
| QB | ND | ND | ND | ND | |
| amoA Abundance | TC | * | ND | ND | * |
| BG | * | ND | ND | * | |
| QB | * | * | 5.6 ± 0.1 | 4.9 ± 0.4 | |
| amoA Expression | TC | ND | ND | ND | ND |
| BG | ND | ND | ND | ND | |
| QB | ND | ND | ND | ND | |
1
Non-numerical labels represent classifications of gene presence based upon a minimum occurrence in 2 of 3 mesocosm replicates.
2
Only compost-amended treatments are represented since gene abundance and expression were below detection limits in the TO treatment for the duration of the experiment.
*
Gene was not detected using real time PCR, but could be detected using a double conventional qPCR as explained in the Materials and Methods
ND: Non-detectable