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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: J Rehabil Res Dev. 2014;51(5):841–854. doi: 10.1682/jrrd.2012.12.0232

Figure 4. pbhImmunofluorescent images of Pax7 in the third parasternal intercostals, from (a.) an ITTO animal and (b.).

Figure 4

Figure 4

a SHAM animal. Arrows show Pax7-positive nuclei, which were defined as areas that fluoresced with Pax7-AlexaFluor-488, were contained within the sarcolemma and basal membrane, and co-localized with DAPI staining. Each image layer was acquired with exposure times of 150-220 msec and gain of 2.0. At least 250 fibers were analyzed with 10X eyepiece and 20X magnification. Scale bar = 100 μm.