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. Author manuscript; available in PMC: 2015 Jun 4.
Published in final edited form as: Nature. 2014 Nov 24;516(7529):116–120. doi: 10.1038/nature13955

Figure 1. TRIM37 is a histone H2A ubiquitin ligase that is over-expressed in 17q23-amplified human breast cancer cell lines.

Figure 1

a, b, In vitro ubiquitination assays with H2A-wild-type (WT) or H2A-KKRR (a) or reconstituted nucleosomes (b). c, (Top) Immunoblots in MCF7 cells expressing a NS, TRIM37 or RNF2 shRNA. (Bottom) Quantification of H2A-ub relative to α-tubulin (TUBA); H2A-ub in NS cells was set to 1. d, Immunoblots in MCF10A cells expressing a NS or RNF2 shRNA. e, qRT-PCR (left) and immunoblots (right) in MCF10A cells expressing TRIM37 or vector. RNF2 expression and H2A-ub levels were normalized to that obtained with vector. Error bars indicate SEM; n=3 technical replicates of a representative experiment (out of three experiments). *P<0.05; **P<0.01.