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. Author manuscript; available in PMC: 2015 Jun 4.
Published in final edited form as: Nature. 2014 Nov 24;516(7529):116–120. doi: 10.1038/nature13955

Figure 3. Interaction and co-occupancy of TRIM37 and PRC2.

Figure 3

a, Immunoblots on sucrose gradient fractions. b, Sucrose gradient fractions 20–22 immunoprecipitated with a TRIM37, EZH2 or SUZ12 antibody, or an IgG control, were analysed by immunoblotting. c–e, Venn diagrams showing overlap between TRIM37- and EZH2-bound genes (c) and TRIM37-bound and H3K27me3-enriched genes in parental MCF7 cells (d) or following knockdown of TRIM37 (e). See also Supplementary Tables 3 and 4. f, BMI1 and EZH2 ChIP in MCF7 cells expressing a NS or TRIM37 shRNA. Error bars indicate SD; n=3 technical replicates of a representative experiment (out of three experiments). g, H3K27me3 ChIP in MCF7 cells. Error bars indicate SD; n=3 technical replicates of a representative experiment (out of three experiments). h, qRT-PCR monitoring TRIM37 target gene expression in BMI1 or EZH2 knockdown MCF7 cells. Error bars indicate SEM; n=3 technical replicates of a representative experiment (out of three experiments). *P<0.05; **P<0.01.