Extended Data Table 2.
List of primers used for quantitative real-time RT-PCR, ChIP and vector construction.
| Gene | Forward primer (5’ → 3’) | Reverse primer (5’ → 3’) | |
|---|---|---|---|
| qRT-PCR | ACTB | ACGTTGACATCCGTAAAGACCT | GCAGTAATCTCCTTCTGCATCC |
| ACTN1 | TCCATCGGAGCCGAAGAAATC | GTGTCGGTGGATCAAAGCACA | |
| ADAM7 | GGTGCCGTATGGTGCCAATTA | CCCCAAATTCGGTTCCTTAGTT | |
| ASS1 | TCCGTGGTTCTGGCCTACA | GGCTTCCTCGAAGTCTTCCTT | |
| BCL2L10 | CGGAAGAAGCGCCATCTCC | AGGCGTCCTTCTTCACTTCAG | |
| BMI1 | TTCTGCTGATGCTGCCAATG | TCCGATCCAATCTGTTCTGGTC | |
| CDT1 | ATGTCAAGGAGCACCACAG | ACTTCATCCACGTTGAAGCG | |
| CKB | ACAACAACCACATGGCCAAG | AGCCCACGGTCATGATGTAC | |
| CUL1 | AACAGTGCAAGTGACGATGC | TTGCTCACGCCAATGTCTTG | |
| CUL3 | TAAACCAACACAGCGGGTTC | TCACCTTGTTTGGCAGCAAC | |
| DDR1 | TTCCCCCTTAATGTGCGTAAGG | AGCAGCCGAATGATGTTTGG | |
| DNMT1 | GAGCTACCACGCAGACATCA | CGAGGAAGTAGAAGCGGTTG | |
| EEF1A1 | GAAGACCCACATCAACATCGT | CTCCGCATTTGTAGATGAGGTG | |
| EGR1 | CAGCACCTTCAACCCTCAGG | GAGATGTCAGGAAAAGACTCTGC | |
| EZH2 | AGTTCGTGCCCTTGTGTGATAG | TGGTGTTGCACTGTGCTTTG | |
| GAPDH | TGCACCACCAACTGCTTAGC | GGCATGGACTGTGGTCATGAG | |
| GRIN1 | ACGCCATCCTAGTTAGCCATC | GCACGGGTATGCGGTAGAAG | |
| HEYL | GGCTGCTTACGTGGCTGTT | GACCCAGGAGTGGTAGAGCAT | |
| IL24 | TTCTGGGCTGTGAAAGACAC | TTTCTTGCGAGACGTTCTGC | |
| IL36A | CCAGACGCTCATAGCAGTCC | AGATGGGGTTCCCTCTGTCTT | |
| JPH3 | AGAATGGGGATGAGCTCAAGTC | TTTTGCTACCGCGACATCTC | |
| LAMP3 | GCGTCCCTGGCCGTAATTT | TGCTTGCTTAGCTGGTTGCT | |
| LTBP3 | TCCCCAGGGCTACAAGAGG | AGACACAGCGATAGGAGCCA | |
| PARD6G | GTCTCAGACCTTGCGATTCTAC | GGTCCAGAGAGAACCTTCGGA | |
| PARP9 | TGGTTGTGGCCATTTCTGTG | ACCATGTTCACCAGCACAAC | |
| PAWR | TGATGAAGCAGGGCAGAAAG | AAACTGTTCTAGGTGGCTCCTG | |
| PLCB3 | TTCATTACCCACGGCTTCAC | TCTTTGCCTGTTGCTTTGCC | |
| PPP1R1B | CAAGTCGAAGAGACCCAACCC | GCCTGGTTCTCATTCAAATTGCT | |
| PROP1 | GCCCAGTGCAGTTGGAACA | TGCTTCCGTTGCTTAGCTCTG | |
| P14ARF | CCCTCGTGCTGATGCTACTG | ACCTGGTCTTCTAGGAAGCGG | |
| RASSF3 | GCCTGCAAGCTCTCAGACC | TAGGCTGTGTAGCGCCTCTT | |
| RASSF9 | GAGGACCTGAGCGAAAGTGAT | TCTGGATGCCACTCAAATGAGA | |
| RNF2 | GTGCAGACAAACGGAACTCA | TTGCCACTTCTAAGGGCTGT | |
| SEPN1 | CCTGACCCTAGCGAGGAGAC | GGCTGTCCAGTTTCGGAGG | |
| SFN | TGACGACAAGAAGCGCATCAT | GTAGTGGAAGACGGAAAAGTTCA | |
| SFRP1 | TCATGCAGTTCTTCGGCTTC | TTGTCACAGGGAGGACACAC | |
| TBKBP1 | GGCGGCTAGAAGAGGCTTTG | CTCCGTCAGCTCGGTGTAG | |
| TP53 | ACTTGTCGCTCTTGAAGCTAC | GATGCGGAGAATCTTTGGAACA | |
| TRAF2 | TGCCCCAAGTTCCCCTTAAC | TTGCCACAAGTCTTGACGTG | |
| TRIM37 | AACAGAGCGTGGAGAGCATT | CTTCTGCCCAACGACAATTT | |
| UNC5B | GTCGGACACTGCCAACTATAC | CCGCCATTCACGTAGACGAT | |
| VCL | AAACTGAACCAGGCCAAAGG | TTTGCCTGCACAGAGTTCAC | |
| WNT10B | GTGAGCGAGACCCCACTATG | CACTCTGTAACCTTGCACTCATC | |
| YES1 | GCCTGTCAGTACAAGTGTGAG | AAAGGCGTTACCCCTGAGGAT | |
| ChIP | Actb (mouse) | CGAAGCCGGTGAGTGAGC | ACCGCCGAGACCGCGTCC |
| ACTB | CCGTTCCGAAAGTTGCCTT | CGCCGCCGGGTTTTATA | |
| ADAM7 | AACTGGGAGCAAGTGAACTG | CTGCTCCAAGGACGACTTTTTC | |
| BCL2L10 | AGGAGGTTGTGGTTCAGACC | ACTTAGCAGCCAGAGTGATCC | |
| CDT1 | GGGATTACAGGCGTGAACCA | ATCCGCTCTCTTGGAACTGC | |
| CKB | CAGGTGTCCCTTAAGCCTGG | CCCTTCAGGTTTTGTGGGGT | |
| CUL1 | CGAGGGAGGCTGTAAAACCTG | CGTGGGGAAGCTTCTACCTG | |
| DDR1 | ACCTGCTTCTTCCTCTGTGC | CCCCAAACGAGAACCTCCAA | |
| DNMT1 | CCACGCCTGGCTAATTTTTG | ATCACGAGGTCAGGAGTTCG | |
| EEF1A | AGCTCGGGATCAAGAATCAC | TGGCCTCTTTACGGGTTATG | |
| EGR1 | CTGGGAGTGGAGAGGGAAC | AATCCGAMGAGGGGCTTCC | |
| Fas (CP/TSS) | GCCGCCTGTGCAGTGGTGA | CTGTGTGTGGGCAGCCTGCGGC | |
| Fas (1 kb) | GGCTATAGATCACCTTCATGTA | GCAGTTAACTCAGGGACCAAG | |
| Fas (2 kb) | GCGTTGCCATAGCATGAACT | GAGTTAGGGGACCATAGTCA | |
| Gapdh (mouse) | CACGCTCGGTGCGTGCCC | GCTCAGCCAGTCCCAGCC | |
| GAPDH | TCCGGGTGATGCTTTTCCTAG | TTTGCGGTGGAAATGTCCTTTTC | |
| IL24 | TTCTTCGCTGTGGACTACTGTC | AACAACACAGAGGTGCTTGG | |
| IL36A | TCAACTATGTGTGGCTTGTGC | GCTAAACTCATTCCACAAGAAGC | |
| JPH3 | GTTTGCAAAGGGCCTCAGTC | AGGACTGCGTCATGGGAAG | |
| LAMP3 | AGCACCTGGACAGCAATTAC | AGGTCAGGTTGTGAGGTGAATG | |
| PARP9 | GTGTGGTTCTGGCTAAATGCAC | TGCGGCACATTTGTACAAGG | |
| PAWR | TGTTGTGGGAAACGTATGGC | TTAGGTAAACAGCCGCTCAGC | |
| PLCB3 | TGTGAAGGTCTCCAGCCATG | ATGGGAACTGGACAGGAATCTG | |
| RASSF3 | TAATCCCAGCTACTTGGGAAGC | TTTTGCTCTGTCACCCAGAC | |
| RASSF9 | ATCATCGCCACCTGGTATCAC | GCAAACAGAGGGAACAACATGG | |
| SEPN1 | AGGCGCAGTGTATGTGTCAC | TCGGAGGAAGGAAATGACAGAC | |
| SFN | ACAGGCCACCAACTTGAAAG | TAACAAGCAGCCTCTGAAGC | |
| SFRP1 | CCCTCCACCTCAAGCCAAAT | ACAAGTCCTCCTGCCATTCG | |
| TP53 | TTGCCAGGAAAGATGCACAG | ATGTGGCAGTTGTGTTGCTC | |
| TRAF2 | GCGCGCTCTTTGAGGAAAAA | CAGCCCTCAGGAAGCTGTAG | |
| WNT10B | TTCTTTCTGCCTCCACACTCTC | AGCACTTTCTCGGAAACCTG | |
| YES1 | TGATGAGGGTGTGAGTAACGC | TGGTTGGCTGTTTGCTTCTC | |
| GAL4 recruitment | GAL4-UAS | GACACTATAGAATCGAGCAGCTG | ATGAATTCGAGCTCGGTACCC |
| TRIM37 | AACAGAGCGTGGAGAGCATT | CTTCTGCCCAACGACAATTT | |
| Vector construction | TRIM37-C18R | CATACGTATGAAACATCGGAAAACCTCAGC | CATACGTATGGAGAAATTGCGGGATG |
| H2A-KKRR (N terminus) | ATGTCTGGTCGTGGCAAACA | ATGCGCGTCCTCCTGTTGTC | |
| H2A-KKRR (C terminus) | (P)-CATCCCTCGTCACCTCCAG | TGGGTGGCTCTAAAAAGAGCC | |
| Cloning TRIM37 into pGEX4T-1 | GAATTCATGGATGAACAGAGCGTGGA | GCGGCCGCTCTTCCACTATTTT | |
| Cloning TRIM37 into pCMX-Ga14 | GGTACCAAATGGATGAACAGAGCGTGGA | GCTAGCTCTTCCACTATTTT | |
| TRIM37ΔRING | GAATTCATGGATGAACAGAGCGTGGAACTCCAGC- TACGAGAACTAGTAAA |
CTCCAGCTACGAGAACTAGTAAA | |
| TRIM37ΔBBOX | (P)-ATGTTTGGTGAGACTGCAGAG | ATTTATGAGCAACACGTCACTAAA | |
| TRIM37ΔCC | (P)-AGTGACGTGTTGCTCATAAATTTCT | GAGTTGATATCTAAGAGCTCAGAGA | |
| TRIM37ΔMATH | (P)-AGTAGCTGAATCGTAAGATGGCA | GCAAATGAAGGATACTTGAATCCACA | |
| TRIM37ΔNLS | (P)-CAAGCCACTGAAAACTGCAACC | ATGGTCACCTTGGGGGCTAAT |