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. 2014 Dec 17;9(12):e114864. doi: 10.1371/journal.pone.0114864

Figure 5. SDS-PAGE of the purified BmrA.

Figure 5

A, 10% SDS-PAGE of 15 µg of purified BmrA in the detergents as indicated in the figure. The numbered bands were cut out and their trypsin digested products were analyzed by LC-MS/MS (see table 1). B, 10% SDS-PAGE of 5 µg of purified BmrA in the detergents as indicated in the figure. *BL21(DE3)ΔacrAB, ΔacrEF was used for the overexpression of BmrA. Please note that samples for FC12 in panels A and B were obtained from two different purifications protocols (see the text).