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Extraction of BmrA using increasing concentrations of FC12. The concentrations of FC12 used were, 0.24% (5×CMC), 0.5%, 0.9%, 1.4%, 2.4% and 3.3% (70×CMC). Extracted material was resolved on a 10% SDS-PAGE at equivalent protein concentrations. Mb: the membrane fraction, Buffer: control without detergent. The Red arrow indicates the position of OmpF which is expected to migrate as a trimer in the conditions used for the electrophoresis [46].
