Table 1. Impurity identification of purified BmrA in the various detergents.
| Detergent | Banda | Score | # of Peptides | Coverage (%) | Mw (kDa) | Accession # | Name |
| FC 12 | 1 (230 kDa) | 2 204 | 59 | 21 | 64.5 | O06967 | BmrA |
| 361 | 13 | 15 | 39.3 | P02931 | Outer membrane protein F (OmpF) | ||
| 2 (180 kDa) | 19 119 | 402 | 44 | 64.5 | O06967 | BmrA | |
| 246 | 4 | 7 | 39.3 | P02931 | OmpF | ||
| 3 (120 kDa) | 14 785 | 442 | 44 | 64.5 | O06967 | BmrA | |
| 6 760 | 235 | 53 | 39.3 | P02931 | OmpF | ||
| 4 (110 kDa) | 510 | 12 | 18 | 64.5 | O06967 | BmrA | |
| 217 | 7 | 15 | 39.3 | P02931 | OmpF | ||
| 5 (85 kDa) | 591 | 19 | 19 | 82.1 | P05825 | Ferrienterobactin receptor (fepA) | |
| 306 | 9 | 13 | 64.5 | O06967 | BmrA | ||
| 116 | 3 | 8 | 39.3 | P02931 | OmpF | ||
| 6 (55 kDa) | 9 119 | 209 | 60 | 64.5 | O06967 | BmrA | |
| 2 164 | 63 | 52 | 82.1 | P05825 | fepA | ||
| 1 163 | 34 | 57 | 39.3 | P02931 | OmpF | ||
| LMNG | 7 (150 kDa) | 429 | 14 | 22 | 64.5 | O06967 | BmrA |
| 8 (110 kDa) | 196 | 4 | 3 | 113.5 | P31224 | Acriflavine resistance protein B (AcrB) | |
| 9 (45 kDa) | 188 | 5 | 10 | 64.5 | O06967 | BmrA | |
| DDM | 10 (150 kDa) | 108 | 2 | 5 | 64.5 | O06967 | BmrA |
| 11 (120 kDa) | 185 | 3 | 5 | 64.5 | O06967 | BmrA | |
| 12 (110 kDa) | 418 | 8 | 7 | 113.5 | P31224 | AcrB | |
| 114 | 2 | 5 | 64.5 | O06967 | BmrA | ||
| LDAO | 13 (250 kDa) | 327 | 14 | 13 | 64.5 | O06967 | BmrA |
| 14 (180 kDa) | 596 | 18 | 20 | 64.5 | O06967 | BmrA | |
| 15 (170 kDa) | 466 | 11 | 13 | 64.5 | O06967 | BmrA | |
| 16 (150 kDa) | 18 727 | 475 | 50 | 64.5 | O06967 | BmrA | |
| 2 428 | 70 | 26 | 34.9 | P0ABJ2 | Cytochrome ubiquinol oxidase sub. 2 | ||
| 297 | 5 | 6 | 74.3 | P0ABJ0 | Cytochrome ubiquinol oxidase sub. 1 | ||
| 17 (110 kDa) | 104 | 3 | 5 | 64.5 | O06967 | BmrA | |
| 18 (75 kDa) | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | |
| UTM | 19 (150 kDa) | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |
| 20 (110 kDa) | 1 836 | 44 | 21 | 113.5 | P31224 | AcrB | |
| 421 | 11 | 8 | 99.6 | P0AFG9 | Pyruvate dehydrogenase E1 component | ||
| 222 | 4 | 3 | 114.6 | Q8FK36 | Cation efflux system protein (CusA) | ||
| 158 | 3 | 3 | 105.0 | P0AFG5 | 2-oxoglutarate dehydrogenase E1 | ||
| 21 (55 kDa) | 15 230 | 427 | 57.2 | 64.5 | O06967 | BmrA | |
| 22 (35 kDa) | 227 | 5 | 16 | 36.3 | A7ZP72 | Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase | |
| UDM | 23 (150 kDa) | 177 | 8 | 7 | 64.5 | O06967 | BmrA |
| 102 | 2 | 13 | 34.9 | P0ABJ2 | Cytochrome ubiquinol oxidase sub. 2 | ||
| 95 | 2 | 8 | 36.3 | A7ZP72 | Undecaprenyl-phospate 4-deoxy-4-formamido-L-arabinose transferase | ||
| 24 (110 kDa) | 3 204 | 65 | 19 | 113.5 | P31224 | AcrB | |
| 1 574 | 51 | 26 | 99.6 | P0AFG9 | Pyruvate dehydrogenase E1 | ||
| 638 | 19 | 11 | 105 | P0AFG5 | 2-oxoglutarate dehydrogenase E1 | ||
| 351 | 9 | 9 | 64.5 | O06967 | BmrA | ||
| 254 | 6 | 5 | 102.0 | A7ZHI9 | Protein translocase subunit (SecA) | ||
| 158 | 3 | 3 | 99.4 | P0ABB9 | Magnesium-transporting ATPase | ||
| 120 | 4 | 4 | 114.6 | Q8FK36 | CusA | ||
| 25 (55 kDa) | 34 422 | 903 | 61 | 64.5 | O06967 | BmrA | |
| DDM* | 26 (150 kDa) | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |
| 27 (110 kDa) | 95 | 5 | 4 | 99.6 | P0AFG9 | Pyruvate dehydrogenase E1 | |
| 90 | 2 | 2 | 105 | P0AFG5 | 2-oxoglutarate dehydrogenase E1 | ||
| 28 (50 kDa) | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |
a
band numbering correspond to the bands cut out from the 10% SDS-PAGE in Fig. 5. The apparent molecular weights as estimated by migration on the gel are indicated in parenthesis. n.d., no protein detected. The score is a protein quality identification index, considering the number of peptide sequences and MS/MS spectra that have been identified for each protein.