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. Author manuscript; available in PMC: 2014 Dec 17.
Published in final edited form as: Mol Endocrinol. 2006 Mar 30;20(8):1825–1837. doi: 10.1210/me.2006-0046

Fig. 1. Analysis of Estrogen-Dependent Regulation of Bip and ERα Proteins in Uteri of Wild-Type Mice.

Fig. 1

Adult ovariectomized wild-type mice were given a single injection (sc) of E2 (100 ng/mouse) and killed at indicated times. A, Uterine tissue extracts were analyzed by Western blotting using the primary antibodies for Bip and actin. B and C, Uterine tissue extracts were immunoprecipitated with Bip-specific primary antibody, and then analyzed by Western blotting using Bip- (B) or ERα- (C) specific antibodies. The intense band detected at approximately 55 kDa in all lanes represents heavy chain subunit of IgG (this shown as an internal loading control). Arrow denotes the position of the detected protein band. In our control experiments, immunoprecipitation using normal goat serum did not detect any specific bands for Bip or ERα by Western blotting (data not shown). D, Uterine tissue extracts were analyzed by Western blotting using the primary antibody for ERα and actin. IP, Immunoprecipitation; WB, Western blot.