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. Author manuscript; available in PMC: 2014 Dec 17.
Published in final edited form as: Mol Endocrinol. 2006 Mar 30;20(8):1825–1837. doi: 10.1210/me.2006-0046

Fig. 5. Analysis of Estrogen-Dependent Water Permeability Responses in Ovariectomized Mice after Administration of Adenoviruses.

Fig. 5

Experimental conditions were same as described in Fig. 4C. A, Analysis of uterine wet weights. Uterine weights were examined at 6 and 24 h after E2 injection. Values with asterisks are statistically different (P< 0.01; ANOVA followed by Newman-Keul’s multiple-range test) as compared with their corresponding control groups. B, Analysis of uterine water uptake. The asterisk indicates statistically different (P < 0.01; ANOVA followed by Newman-Keul’s multiple-range test) as compared with control group. C, Analysis of uterine macromolecular uptake. The fluorescence for the uptake of rhodamine-BSA in uterine tissue sections was visualized by direct fluorescence (panels b and d) and phase-contrast (panels a and c) microscope. le, Luminal epithelium; s, stroma. Bars, 100 µm. D, Analysis of uterine permeability gene expression. Frozen uterine sections were analyzed by in situ hybridization of VEGF and Flk-1. le, Luminal epithelium; myo, myometrium; s, stroma. Bars, 100 µm. VEGF, Vascular endothelial growth factor; Flk-1, VEGF receptor 2.