Figure 2. Warts regulates lymph gland size and hemocyte proliferation.

(A–C) Direct interference contrast images of control w¹¹¹⁸ (A) and wts mutant late third instar lymph glands (B). Lymph gland size was quantified in (C). wts mutant lymph glands were significantly larger than controls (p<0.0001; n=30 for w¹¹¹⁸, n=16 for wts-Z; error bars represent SEM).

(D–L) Control w¹¹¹⁸ (D, G and J) and wts mutant lymph glands (E, H and K) at late second instar (D–E), early third instar (G–H) and mid third instar (J–K). Cells undergoing DNA replication were visualised using EdU (red in D, E, G, H, J and K) and nuclei were marked with DAPI (blue in D, E, G, H, J and K). The number of EdU positive cells per lymph gland was quantified in (F, I and L).

(M–O) Control w¹¹¹⁸ (M) and wts (N) mutant mid third instar larval lymph glands. Mitotic cells were visualised using anti phospho-Histone H3 (white) and nuclei were marked with DAPI (blue). The number of phospho-Histone H3 positive cells per lymph gland was quantified in (O).

Scale bars = 20 μm. See also Figure S2.