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c-Fos-specific immunohistochemistry in the area postrema (AP) and nucleus tractus solitarius (NTS) of rats at 0 (A), 0.5 (B), 1 (C), 2 (D), 4 (E), 8 (F), and 16 h (G) after gavage with distilled water. Few c-Fos-positive nuclei were observed in the NTS 0 h after gavage. The number of c-Fos-positive nuclei (arrows) was increased 1~2 h after gavage and decreased thereafter over time. Scale bar = 50 µm.
