Fig. 4.

Effect of miR-766 on hCyp11B2 mRNA level in human adrenal (H295R) cells. H295R cells were transfected either in the absence of miRNA (mock) or in the presence of mut miR-766 or miR-766 miRNA mimics (50 nM). After 48 h, transfected cells were washed twice with ice-cold 1× PBS, and total RNA was isolated and reverse-transcribed into cDNA. qRT-PCR was performed; cycle threshold (ct) values for hCyp11B2 were determined and normalized to GAPDH expression. Relative ct values for each group were determined and compared with those for mock transfections taken as 1 for fold change. Each group represents the mean of at least 3 independent experiments. *P < 0.05.