Fig. 2.

Pharmacological inhibition of PKG with DT-2 did not have any effect on the TBKCs in freshly isolated UBSM cells. A: an original voltage clamp recording illustrating that DT-2 (1 μM) did not affect TBKCs and that the subsequent PKA inhibition with H-89 completely inhibited TBKCs in a UBSM cell. A portion of the recording under each condition is shown on an expanded time scale. B: summary data illustrating that DT-2 (1 μM) did not have a significant effect on the frequency (Freq) and amplitude (Amp) of TBKCs (n = 6, N = 5). P > 0.05.