Fig. 4.

Pharmacological inhibition of PKA with H-89 increased the global cellular Ca²⁺ levels and the magnitude of Ca²⁺ oscillations in freshly isolated UBSM cells. A: an original recording illustrating that H-89 increased the global Ca²⁺ levels in a freshly isolated UBSM cell. B: summary data show that the inhibition of PKA significantly increased the global intracellular Ca²⁺ levels in UBSM cells (n = 8, N = 4). C: the overlay of 100 s of recordings under control conditions and after H-89 treatment shows that H-89 increased the oscillation of the cytosolic Ca²⁺ in UBSM cells. D: summary data show that the inhibition of PKA significantly increased the magnitude of Ca²⁺ oscillations, which is measured as the standard deviation (SD) of the F₃₄₀/F₃₈₀ ratio during the last 5 min of recording in the absence and presence of H-89 (10 μM; n = 8, N = 4). *P < 0.05. Con, control.