Fig. 1.

Long-term treatment with Bifidobacterium sp. culture supernatant (CS) stimulates Cl⁻/HCO₃⁻ exchange activity in Caco-2 cells. Fully differentiated Caco-2 cells were treated with a 1:2 dilution of Bifidobacterium breve Bifidobacterium infantis, or Bifidobacterium bifidum CS for 24 h (A) or with different dilutions of B. breve CS (B), and apical Cl⁻/HCO₃⁻ exchange activity (DIDS-sensitive ¹²⁵I⁻ uptake) was measured. Values are means ± SE of 3–4 separate experiments. *P < 0.05, **P < 0.01 vs. control.