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High-fat feeding alters cytokine mRNA expression. Quantitative real-time PCR analysis of cytokine expression in adipose tissue (A) and liver (B) of mice fed an HFD or an LFD. Expression levels were normalized to 36B4 in adipose and liver. Expression levels in liver were normalized to β-actin. Data shown are means ± SE (n = 7–12 per group). *P < 0.05; **P < 0.01.
