Table 1.
Primers used in real-time PCR analysis
| Gene | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) |
|---|---|---|
| g-Csf | ATGGCTCAACTTTCTGCCCAG | CTGACAGTGACCAGGGGAAC |
| Il-22 | ATGAGTTTTTCCCTTATGGGGAC | GCTGGAAGTTGGACACCTCAA |
| Il-16 | AAGAGCCGGAAATCCACGAAA | GTCTCAAAAGGGTCAGGGTACT |
| Il-1α | GCACCTTACACCTACCAGAGT | AAACTTCTGCCTGACGAGCTT |
| Il-1β | GTGGCTGTGG AGAAGCTGTG | GAAGGTCCACGGGAAAGACAC |
| Cxcl1 | CTGGGATTCACCTCAAGAACATC | CAGGGTCAAGGCAAGCCTC |
| Il-1R1 | GTGCTACTGGGGCTCATTTGT | GGAGTAAGAGGACACTTGCGAAT |
| Il-2Rα | AACCATAGTACCCAGTTGTCGG | TCCTAAGCAACGCATATAGACCA |
| Vegf3 | CTGGCAAATGGTTACTCCATGA | ACAACCCGTGTGTCTTCACTG |
| Lcn2 | TGGCCCTGAGTGTCATGTG | CTCTTGTAGCTCATAGATGGTGC |
| Ptx3 | CCTGCGATCCTGCTTTGTG | GGTGGGATGAAGTCCATTGTC |
| Timp-1 | GCAACTCGGACCTGGTCATAA | CGGCCCGTGATGAGAAACT |
| Sap | AGACAGACCTCAAGAGGAAAGT | AGGTTCGGAAACACAGTGTAAAA |
| Cd30 | CCTTCCCAACGGATCGACC | CCCGTCTTCATTGACGTAGTAGT |
| Il-12p40 | TGGTTTGCCATCGTTTTGCTG | ACAGGTGAGGTTCACTGTTTCT |
| Ccl12 | ATTTCCACACTTCTATGCCTCCT | ATCCAGTATGGTCCTGAAGATCA |
| Cx3cl1 | ACGAAATGCGAAATCATGTGC | CTGTGTCGTCTCCAGGACAA |
| Ccl4 | TTCCTGCTGTTTCTCTTACACCT | CTGTCTGCCTCTTTTGGTCAG |
| Ccl20 | CGTCATCCATGGCGAACTG | GCTTCTTTGCAGCTCCTTCGT |
| Rage | CTTGCTCTATGGGGAGCTGTA | GGAGGATTTGAGCCACGCT |
| Saa-3 | TGCCATCATTCTTTGCATCTTGA | CCGTGAACTTCTGAACAGCCT |
| Haptoglobin | GCTATGTGGAGCACTTGGTTC | CACCCATTGCTTCTCGTCGTT |
| A2M | AGATGGTGAGATTTCGTGTTGTC | ACGGTCCTGCCTGATTCTGTA |
| Npy | ATGCTAGGTA ACAAGCGAATGG | TGTCGCAGAGCGGAGTAGTAT |
| Agrp | ATGCTAGGTAACAAGCGAATGG | CAGACTTAGACC TGGGAACTCT3 |
| Pomc | TGCCGAGATTCTGCTACAG | TGCTGCTGTTCCTGGGGC |
| Cart | CCCGAGCCCTGGACATCTA | G CTTCGATCTGCAACATAGCG |
| β2M | TTCTGGTGCTTG TCTCACTGA | CAGTATGTTCGGCTTCCCATTC |
| 36B4 | AGATTCGGGATATGCTGTTGGC | TCGGGTCCTAGACCAGTGTTC |
| β-actin | GGCACCACACCTTCTACAATG | GGGGTGTTGAAGGTCTCAAAC |