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Relaxin does not increase dimethylarginine dimethylaminohydrolase (DDAH) abundance or activity in the kidney cortex or liver. Western blot analysis of expression of DDAH isoforms (A-D) and total DDAH enzymatic activity (E-F) in the kidney cortex and liver from CON, RLX, ANG, and ANG+RLX rats. DDAH activity is expressed as μmol citrulline produced per g of protein per min; n = 6–8. *P < 0.05 vs. control. G: representative Western blots for the expression of DDAH-1 and DDAH-2.
