Fig. 5.

Renal tubule cell damage after photoablation injury is greater in zebrafish following knockdown of exocyst sec10. Following injection into 1-cell stage embryos of control and splice-site morpholinos (MO) that we had previously shown to efficiently knock down sec10 in zebrafish embryos (9), laser photoablation was performed in EGFP-expressing segments of ET11-9 zebrafish kidney tubule epithelium. The arrows in A point to the boundary between the ablated and intact epithelium. B: Sec10 morphants (n = 8), compared with control morphants (n = 11), showed a significantly greater reduction in GFP positivity and, therefore, greater injury in the photoablated segment at three h following injury (P = 0.034). The %remaining GFP intensity was determined by comparing the average pixel intensity in the injured segment to the average pixel intensity in the “bracketing” intact segments, and statistical comparison was performed via two tail unpaired t-test.