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CD-31 staining (brown stain) in the LV of control and Sirt1endo−/− animals at different ages. Quantification of the CD31 staining is presented in the bar graph as the number of vessels per square millimeter. Note that the capillary density in the LV myocardium of the Sirt1endo−/− mice was decreased after 30–40 wk of age compared with control mice.
