Fig. 7.

Intrinsic properties of bushy cells. A: superimposed responses to depolarizing and hyperpolarizing current pulses from a bushy cell from a deaf Otoferlin mutant mouse. B: average input resistances from bushy cells in hearing and deaf mice calculated from responses to 10 pA and −10 pA current steps were not significantly different between hearing and deaf Otoferlin mutant mice. C: average resting membrane potentials in bushy cells were not significantly different between hearing and deaf mice. D: rate-of-depolarization (dV/dt) threshold was significantly lower in the bushy cells of deaf than of hearing mice, indicating that bushy cells from hearing mice require a steeper depolarization to fire action potentials than bushy cells from deaf mice. E: representative current-clamp recording from a hearing mouse illustrates how ramped depolarizing current pulses injected into the bushy cell were used to measure the rate-of-depolarization thresholds. Black traces are the responses to the current ramp that were just suprathreshold and just subthreshold. The slope of the black suprathreshold voltage trace was the rate threshold. *Statistically significant difference.