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. 2014 Sep 24;34(12):1898–1906. doi: 10.1038/jcbfm.2014.158

Figure 2.

Figure 2

Oxygen/glucose deprivation (OGD) causes differential activation of Rac1, but not Cdc42, in cortical and hippocampal neurons. (A) Cell lysates from control conditions or after 20 minutes of OGD were incubated with GST-PAK1 immobilized on glutathione-agarose beads to isolate active (GTP-bound) Rac1. Cell lysates from control conditions were also incubated with GST as a negative control. Representative blots show the levels of active (PAK1-bound) and total Rac1 (~21 kDa) in hippocampal and cortical neurons under control and OGD conditions. Graph shows pooled data presented as mean±s.e.m. The proportion of active Rac1 increased in hippocampal neurons and decreased in cortical neurons in response to OGD. *P<0.05 (T-test). Hippocampal neurons, n=9 independent cultures; cortical neurons, n=11 independent cultures. (B) The same experiment was performed to analyze active Cdc42. Representative blots show a decrease in Cdc42 (~21 kDa) activation in response to OGD in both hippocampal and cortical neurons. *P<0.05 (T-test). Hippocampal neurons, n=7 independent cultures; Cortical neurons, n=5 independent cultures. GST, Glutathione-S-transferase.