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. 2014 Sep 3;34(11):1826–1836. doi: 10.1038/jcbfm.2014.152

Figure 2.

Figure 2

MicroRNA-181a regulated the integrity and permeability of blood–tumor barrier (BTB), and the expression of tight junction-related proteins in glioma endothelial cells (GECs). (A) The transendothelial electric resistance (TEER) values of BTB with the expression of miR-181a changed. (B) The permeability coefficient values of Lucifer Yellow (LY) (457 Da), 4 kDa fluorescein isothiocyanate (FITC)-dextrans, and 70 kDa FITC-dextran in BTB with the expression of miR-181a changed. (C) Quantitative RT-PCR analysis of tight junction-related proteins in GECs with the expression of miR-181a changed. (D) Western blot analysis of tight junction-related proteins in GECs with the expression of miR-181a changed, using GAPDH as an endogenous control. Representative protein expression and their integrated light density values (IDVs) are shown. Data represent means±s.d. (n=5, each). *P<0.05 versus miR-181a (+)-NC group, #P<0.05 versus miR-181a (−)-NC group, △P<0.05 versus miR-181a (+) (#1) group, †P<0.05 versus miR-181a (−) (#1) group. (E) Immunofluorescence staining of tight junction-related proteins in GECs with the expression of miR-181a changed. Nuclei were labeled with DAPI. Images are representative of independent experiments (n=4). Scale bars represent 20 μm. NC, negative control.