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. 2014 Sep 3;34(11):1826–1836. doi: 10.1038/jcbfm.2014.152

Figure 5.

Figure 5

MicroRNA-181a (miR-181a) regulated the integrity and permeability of blood–tumor barrier (BTB), and the expression of tight junction-related proteins in glioma endothelial cells (GECs) by regulating Kruppel-like factor 6 (KLF6). (A) The transendothelial electric resistance (TEER) values of BTB with the expression of miR-181a and KLF6 changed. (B) The permeability coefficient values of Lucifer Yellow (LY) (457 Da), 4 kDa fluorescein isothiocyanate (FITC)-dextrans, and 70 kDa FITC-dextran in BTB with the expression of miR-181a and KLF6 changed. (C) Quantitative RT-PCR analysis of tight junction-related proteins in GECs with the expression of miR-181a and KLF6 changed. (D) Western blot analysis of tight junction-related proteins in GECs with the expression of miR-181a and KLF6 changed, using GAPDH as an endogenous control. Representative protein expression and their integrated light density values (IDVs) are shown. Data represent means±s.d. (n=5, each). *P<0.01 versus control group, ▴P<0.05 versus pre-miR-181a+KLF6 (+) group, #P<0.05 versus anti-miR-181a+KLF6 (+) group, △P<0.05 versus pre-miR-181a+KLF6(−) (#1) group, †P<0.05 versus anti-miR-181a +KLF6(+) (#1) group, and ‡P<0.05 versus anti-miR-181a+KLF6(−) (#1) group.