Figure 2.

(a and b) Temperature- and pressure-dependent FTIR data for (a) G-actin and (b) B-actin. (A and B) Changes in secondary structure elements by pressure at a selected temperature (A) and temperature-dependent changes at ambient pressure (B). (C and D) Example of pressure- (C) and temperature- (D) dependent changes in the deconvoluted absorption signal of the amide I′ band (see Supporting Material for additional data). Lines are Boltzmann fits to the experimental data using Eqs. 1 and 2. Black box, α-helix (1655 cm⁻¹); red circle, intramolecular β-sheets (1635 cm⁻¹); blue up triangle, intramolecular β-sheets (1627 cm⁻¹); green down triangle, intermolecular β-sheets (1616 cm⁻¹). To see this figure in color, go online.