Figure 3.
(A) Calorimetric studies of G-, B-, and F-actin. DSC thermograms (ΔCp with respect to solvent) of the various actin structures display differences in thermal stability. (B) Deconvolution analysis of DSC peaks reveals a biphasic unfolding process for G-actin and a more complex triphasic behavior in the case of B- and F-actin. PPC measurements show temperature-dependent changes in the coefficient of thermal expansion. (C) Integrating the area of α(T) over the transition region yields the volume change of the transition at ambient pressure for the various actin species. To see this figure in color, go online.