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. 2014 Dec 16;107(12):2972–2981. doi: 10.1016/j.bpj.2014.10.062

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© 2014 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/).

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Correlation of relaxation dispersion and titration chemical shifts. Left: plot of the fitted relaxation-dispersion-derived chemical shift differences (Δω_disp) versus the measured ¹⁵N backbone chemical shift differences between apo Bet v 1.0101 and SDS-bound Bet v 1.0101 (Δω_bind). Right: ¹H-¹⁵N-HSQC titration of Bet v 1.0101 with SDS. Spectra are shown for protein/ligand ratios between 1:0 (orange) and 1:3 (black). To see this figure in color, go online.