Figure 2.

Comparison of the temperature dependence of CcO activity with the DSC-monitored thermal transitions. Experimental data are shown as symbols: DSC (solid circles) and CcO activity (open squares). The solid line represents the percent of the native state population, γ_N, calculated using Eq. 6 and the parameters obtained from fitting of the DSC thermal transition data. The coincident decrease of CcO activity and the population of the CcO native state indicate that temperature-induced conformational changes occurring during the first thermal transition are critical for CcO activity. The measurements were performed at the same conditions as described in Fig. 1.