Figure 5.
Subunit composition of CcO before and after the first thermal transition. Nuclear-encoded CcO subunits were analyzed by C18 reversed-phase HPLC (main figure); mitochondrial-encoded subunits were analyzed by SDS-PAGE (inset). Chromatograms labeled as peak A, B, and C represent HPLC of fractions collected under peaks A, B, and C from Fig. 4, respectively. Chromatograms for peaks A and B were those obtained for intact CcO (13-subunit form of CcO) and PL-free CcO (11-subunit form of CcO) before each being heated above 25°C. Chromatogram for peak C was that obtained for PL-containing CcO after it had been heated to 54°C. An identical chromatogram was obtained for peak C material obtained from PL-free CcO that had been heated to 52°C. Analysis of peak C material, whether it had been isolated from either PL-containing or PL-free, had an identical subunit composition. Inset: SDS-PAGE analysis of mitochondrial-encoded CcO subunits composition of intact, PL-containing CcO before (peaks A and B) and after heating to 54°C (peak C). Identical SDS-PAGE results were obtained for peak B and C material that had been isolated from PL-free CcO.