Figure 5.

Diffusion analysis of the Paxillin-EGFP in CHOK1 cells acquired using the point-scan method. (A) Fluorescence intensity map of Pax-EGFP. Bright spots represent the focal adhesion sites. (B–D) Diffusion map of the cell (B) color-coded to match the cursor colors in the phasor plot (D) and representative short decay correlation function (C). (E) Diffusion histogram calculated based on a 0.4 μm ω₀ and 3D diffusion profile. (F) 3D intensity map of the cell, where the intensity was used as the wire frame. The height in the z plane represents intensity. The image was then color-coded to match the cursors in D. The color of the peaks is mostly green, representing slow diffusion in the focal adhesion sites. To see this figure in color, go online.