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Experimental setup overview for the validation of the hits obtained from the genome-wide shRNA screening
| Genome-wide screening | Validation 1 | Validation 2 | |
|---|---|---|---|
| Cells | HeLa CD4++ cl1022 | HeLa CD4++ cl1022 | SupT1 |
| Format | Pooled shRNA library | Single shRNA | Single shRNA |
| shRNA plasmid | pSIF H1 SBI (System Biosciences) | pLKO.1 (MISSION® Sigma Aldrich) | pLKO.1 (MISSION® Sigma Aldrich) |
| CD4 down-modulation inducer | LZRS-NA7 Nef-IRES-eGFP | LZRS-NA7 Nef-IRES-eGFP | HIV-1 NL4.3 eGFP |
