Table 1. All high confidence interactions found in this study. Previously published interactions are in bold with their source citations given under “Ref”.

EHEC effector		Interacting human protein
Name	Sakai gene id	Name	GeneID	Protein names	Ref
EspB	ECs4554	RBCK1	10616	RanBP-type and C3HC4-type zinc finger-containing protein 1
EspB	ECs4554	STK16	8576	Serine/threonine-protein kinase 16
EspF1	ECs4550	MAD2L2	10459	Mitotic spindle assembly checkpoint protein MAD2B
EspF1	ECs4550	SNX33	257364	Sorting nexin-33
EspF1	ECs4550	SNX9	51429	Sorting nexin-9	35
EspG	ECs4590	“Hs.658052”	UGID:2727370	N/A
EspG	ECs4590	NMI	9111	N-myc-interactor
EspJ	ECs2714	CENPH	64946	Centromere protein H
EspJ	ECs2714	IFT20	90410	Intraflagellar transport protein 20 homolog
EspJ	ECs2714	MRFAP1L1	114932	MORF4 family-associated protein 1-like 1
EspJ	ECs2714	RIC8A	60626	Synembryn-A
EspO1-1	ECs1567	FEM1B	10116	Protein fem-1 homolog B
EspY1	ECs0061	CAPN3	825	Calpain-3
EspY1	ECs0061	CDKN2AIPNL	91368	CDKN2AIP N-terminal-like protein
EspY1	ECs0061	CLK1	1195	Dual specificity protein kinase CLK1
EspY1	ECs0061	DNAJC14	85406	DnaJ homolog subfamily C member 14
EspY1	ECs0061	PCID2	55795	PCI domain-containing protein 2
EspY1	ECs0061	PIH1D1	55011	PIH1 domain-containing protein 1
EspY1	ECs0061	PSMC1	5700	26S protease regulatory subunit 4
EspY1	ECs0061	ZNHIT1	10467	Zinc finger HIT domain-containing protein 1
Map	ECs4562	RHPN1	114822	Rhophilin-1
Map	ECs4562	SLC9A3R2	9351	Na(+)/H(+) exchange regulatory cofactor NHE-RF2 (NHERF-2)	34
NleA	ECs1812	DSCR4	10281	Down syndrome critical region protein 4
NleA	ECs1812	FRMD3	257019	FERM domain-containing protein 3
NleA	ECs1812	PENK	5179	Proenkephalin-A
NleA	ECs1812	PTP4A1	7803	Protein tyrosine phosphatase type IVA 1
NleB1	ECs3857	DRG2	1819	Developmentally-regulated GTP-binding protein 2
NleB1	ECs3857	LRRC18	474354	Leucine-rich repeat-containing protein 18
NleB1	ECs3857	POLR2E	5434	DNA-directed RNA polymerases I, II, and III subunit RPABC1
NleC	ECs0847	C8orf71	26138	Putative uncharacterized protein encoded by LINC00588
NleC	ECs0847	CTDSPL2	51496	CTD small phosphatase-like protein 2
NleD	ECs0850	METTL2A	339175	Methyltransferase-like protein 2A
NleF	ECs1815	CASP9	842	Caspase-9	23
NleF	ECs1815	DHFR	1719	Dihydrofolate reductase
NleF	ECs1815	ERI3	79033	ERI1 exoribonuclease 3
NleF	ECs1815	HMGN2	3151	Non-histone chromosomal protein HMG-17
NleF	ECs1815	LMO4	8543	LIM domain transcription factor LMO4
NleF	ECs1815	TRNT1	51095	CCA tRNA nucleotidyltransferase 1, mitochondrial
NleH1-2	ECs1814	UFC1	51506	Ubiquitin-fold modifier-conjugating enzyme 1
TccP	ECs2715	ZNF626	199777	Zinc finger protein 626
Tir	ECs4561	ARRB1	408	Beta-arrestin-1
Tir	ECs4561	BAIAP2	10458	Brain-specific angiogenesis inhibitor 1-associated protein 2	15
Tir	ECs4561	BAIAP2L1	55971	Brain-specific angiogenesis inhibitor 1-associated protein 2-like protein 1	14
Tir	ECs4561	HPCAL1	3241	Hippocalcin-like protein 1
Tir	ECs4561	HPCAL4	51440	Hippocalcin-like protein 4
Tir	ECs4561	NCALD	83988	Neurocalcin-delta
Tir	ECs4561	PDE6D	5147	Retinal rod rhodopsin-sensitive cGMP 3′,5′-cyclic phosphodiesterase subunit delta
Tir	ECs4561	STK16	8576	Serine/threonine-protein kinase 16