Table 2.

Engineered strains for diols production

Strains	Genes/Enzymes	Substrate	Methods	Productivity/Yield	Reference
1,3-Propanediol
Clostridium acetobutylicum DG1(pSPD5)	Glycerolkinase, Glycerol-3-phosphate dehydrogenase	Glycerol	Continuous cultures	-	[65]
Recombinant E. coli	Glycerol3-phosphate dehydrogenase, glycerol 3-phosphate phosphatase, Insert dhaB1-3, yqhD, Delete tpi	Glucose	Fed-batch fermentation	4 g/l/h	[8]
1,2-Propanediol
Escherichia coli	Methylglyoxalsynthase, Glycerol dehydrogenase, Aldehyde oxidoreductase, ATP-dependentdihydroxyacetone kinase	Glycerol	Multi-fermentation	0.21 g/g	[41]
Saccharomyces cerevisiae(sJDPMG)	Overexpress genes:gdh,GUP1, mgs, gldA	Glycerol	Flask cultivation	0.21 ± 0.02 g/g	[45]
Synechococcus elongates PCC 7942	mgsA, yqhD, adh	CO2	Light condition	--	[26]
Corynebacterium glutamicum	Overexpress genes:mgs, cgR_2242	Glucose	Flask cultivation	20 mg/l/h	[38]
Pichia pastoris	Introduce genes:mgsA, yqhD, glydh	Glycerol	Fed-batch fermentation	2 mg/l/h	[70]
2,3-Butanediol
SerratiamarcescensH303	swrW	Sucrose	Fed-batch culturing	3 g/l/h	[56]
Bacillus licheniformis	Knock out theldh	Glucose/Xylose	Anaerobic fermentation	0.45 g/g (BL5 glucose), 0.44 g/g(BL8 xylose)	[51]
Escherichia coli SGSB03	Introduce genes: budA, meso-budC	Glucose/Glycerol	Batch culture	0.31 g/g (glucose)	[15]
				0.21 g/g (glycerol,)
Escherichia coli MGLAP	Acetoacetate synthase, Acetoacetatedecarboxylase, Secondary alcohol dehydrogenase	Cellodextrin	Anaerobic cultivation	0.84 g/g	[19]
1,4-Butanediol
Escherichia coli	Knock out genes: adhE, pflB, ldhA, Replace lpdA		Fed-batchfermentation	0.16 g /l/h	[58]