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. 2014 Dec 3;7:554. doi: 10.1186/s13071-014-0554-y

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© Tonk et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Expression of Scapularisin-3 and Scapularisin-6 in tick tissues and cell lines. The expression pattern determined by PCR and visualised by agarose gel electrophoresis of Scapularisin-3 and Scapularisin-6 in I. scapularis salivary glands (SG), midgut (MG) and embryo-derived tick cell lines ISE6 and ISE18 is shown. Both defensins were expressed in SG. Scapularisin-6 was expressed in MG and neither of the two defensins was expressed by embryo-derived tick cells (ISE6 and ISE18) in vitro. (β-actin was used as a positive control).