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Analysis of alizarin red staining during osteogenic culture of HF-hMSCs on Ti discs and Ti screws. HF-hMSC populations were seeded onto Ti discs or Ti screws and cultured for up to 28 days in osteogenic medium. Matrix mineralisation on Ti surfaces was assessed by staining with alizarin red at days 1, 7, 14, 21 and 28 post-seeding. Scale bars = 1 cm.
