Table 1.
Potential novel biomarkers for Alport syndrome
| Phosphospot | Phosphoprotein identified in the spot | Quantitative value (Normalized total spectra) | Molecular weight, kDa | Isoelectric point, p I | |||
|---|---|---|---|---|---|---|---|
| (Entry Name) | WT/PLC/MMF | Observed | Calculated | Observed | Basal, unphosphorylated state a | Prediction for various phosphorylation states a | |
| Phosphospot 1 | 17 | 9.0 | |||||
| Actin-related protein 2/3 complex subunit 3 (ARPC3_MOUSE) | 9/13/53 | 21 | 8.78 | 1-18 phosphosites; 8.28-4.09 | |||
| Main protein | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (NDUA8_MOUSE)b | 35/49/53 | 20 | 8.76 | 1-11 phosphosites; 8.41-5.45 | ||
| Phosphospot 2 | 75 | 5.5 | |||||
| Plastin-3 (PLST_MOUSE) | 2/0/0 | 71 | 5.42 | 1-44 phosphosites; 5.36-4.19 | |||
| Heat shock cognate 71 kDa protein (HSP7C_MOUSE) | 2/0/0 | 71 | 5.37c | 1-137 phosphosites; 5.32-2.38c | |||
| Adseverin (ADSV_MOUSE) | 5/18/58 | 80 | 5.64 | 1-17 phosphosites; 5.57-4.93 | |||
| Main protein | Serum albumin (ALBU_MOUSE) | 33/13/99 | 69 | 5.75 | 1-120 phosphosites; 5.69-2.63 | ||
| Phosphospot 3 | 45 | 7.5 | |||||
| ATP synthase subunit alpha, mitochondrial (ATPA_MOUSE) | 16/0/0 | 60 | 9.22 | 1-79 phosphosites; 9.07-3.10 | |||
| Catalase (CATA_MOUSE) | 2/0/0 | 60 | 7.72 | 1-69 phosphosites; 7.19-3.73 | |||
| Main protein | Glutamate dehydrogenase 1, mitochondrial (DHE3_MOUSE) | 43/22/37 | 61 | 8.05 | 1-82 phosphosites; 7.40-3.33 | ||
| Phosphospot 4 | 27 | 6.5 | |||||
| Serine/arginine-rich splicing factor 1 (SRSF1_MOUSE) | 4/0/0 | 28 | 10.37 | 1-58 phosphosites; 10.22-3.08 | |||
| 26S proteasome non-ATPase regulatory subunit 9 (PSMD9_MOUSE) | 4/0/0 | 25 | 6.00 | 1-14 phosphosites; 5.83-4.58 | |||
| Main protein | Estradiol 17-beta-dehydrogenase 8 (DHB8_MOUSE)b | 9/30/17 | 27 | 6.10 | 1-5 phosphoprotein; 5.81-5.07 | ||
| Phosphospot 5 | 15 | 8.5 | |||||
| Destrin OS (DEST_MOUSE) | 15/41/10 | 19 | 8.14 | 1-30 phosphosites; 7.15-3.20 | |||
| 40S ribosomal protein S14 (RS14_MOUSE) | 5/0/56 | 16 | 10.07 | 1-15 phosphosites; 9.84-5.26 | |||
| Main protein | Peptidyl-prolyl cis-trans isomerase A (PPIA_MOUSE) | 72/30/44 | 18 | 7.73 | 1-39 phosphosites; 6.75-2.36 | ||
| Phosphospot 6 | 26 | 7.0 | |||||
| Serine/arginine-rich splicing factor 1 (SRSF1_MOUSE) | 12/1/27 | 28 | 10.37 | 1-58 phosphosites; 10.22-3.08 | |||
| Main protein | Omega-amidase NIT2 (NIT2_MOUSE)b | 49/29/34 | 31 | 6.44 | 1-13 phosphosites; 5.10-4.58 | ||
a http://www.phosphosite.org; January 2014.
bMain protein identified in each group, not yet described as phosphoprotein.
cInformation for the human protein; March 2014.
The table presents possible phosphoproteins, including the main proteins identified in this mouse model for the human Alport syndrome.