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. 2014 Dec 16;3:e04147. doi: 10.7554/eLife.04147

Figure 1. Detailed glomerular innervations of excitatory and inhibitory projection neurons in the AL.

(A) Simultaneous labeling of inhibitory projections neurons (iPNs, labeled by MZ699-GAL4;G-CaMP) and excitatory projection neurons (ePNs, labeled by GH146-QF;tdTomato) in vivo reveals distinct projections to the lateral horn (LH). All iPNs bypass the mushroom body calyx (MBc) and innervate the LH exclusively. The MZ699 line labels a few ventrolateral protocerebral neurons (vlPr neurons) projecting via the posterior lateral fascicle (plF) from the ventrolateral protocerebrum (vlPr) to the LH. (B) Schematic of the PN connectivity relay from the antennal lobe (AL) to higher brain centers (ePNs in magenta, iPNs in green, and vlPr neurons in orange). (C) Above, complete glomerular assignment of the AL neuropil (right AL), labeled with elav-n-synaptobrevin:DsRed (END1-2). Below, glomerular innervations of both PN populations related to in vivo images in Figure 1—figure supplement 2. Depicted are the ventral level (∼−40 µm), the medial level (∼−20 µm) and the dorsal view onto the AL. Color annotation: blue glomeruli are not innervated by any of the used GAL4-lines; green glomeruli are innervated by MZ699+ iPNs and magenta by GH146+ ePNs; white glomeruli are innervated by both enhancer trap lines. Scale bar, 20 µm.

DOI: http://dx.doi.org/10.7554/eLife.04147.003

Figure 1.

Figure 1—figure supplement 1. Characterization of excitatory and inhibitory projection neurons.

Figure 1—figure supplement 1.

Overlap of ePNs (QUAS-tdTomato) and iPNs (UAS-GCaMP3.0) in the LH area. The circle indicates the posterior lateral region, which is sparsely innervated by iPNs and dominated by ePN axonal terminal fields. Scale bar, 20 µm.
DOI: http://dx.doi.org/10.7554/eLife.04147.004
Figure 1—figure supplement 2. Glomerular innervations of ePNs and iPNs.

Figure 1—figure supplement 2.

(A) Representative in vivo images of glomerular innervations. MZ699- and GH146-GAL4 lines have been reconstructed with the END1-2 background staining (two upper planes) and dual labeling via the Q-system and the GAL4-UAS expression system (lowest plane). Scale bar, 20 µm. (B) Detailed glomerular AL innervation. Green filled cells indicate innervation by MZ699-GAL4, magenta GH146-GAL4 innervation, respectively and grey, no innervation by the indicated line. Bottom rows, total number of innervated glomeruli with percentage share indicated below. Merge column: white filled with ‘x’ indicates glomeruli innervated by both lines, grey only one line. Blue filled rows are glomeruli labeled by none of the enhancer trap lines.
DOI: http://dx.doi.org/10.7554/eLife.04147.005