Figure 5. Expression of TH-PLE and CATSUP in Drosophila female reproductive system.

(A–C) Confocal images showing striking accumulation (arrowhead) of TH-PLE (green, anti-TH) in D. sechellia (14021–0248.25) (B) and D. melanogaster DGRP-357 (C), compared to in D. melanogaster wild-type Berlin (wtB) (A) oocytes. (D–F) Confocal images showing CATSUP (magenta, anti-SLC39A7) expressed in the nurse cells (arrow) of D. melanogaster wild-type Berlin (wtB) oocytes (D) and absent in the nurse cells of D. sechellia (14021–0248.25, sec) (E) and D. melanogaster DGRP-357 (DGRP-357) (F) oocytes. (G–I) Confocal image showing CATSUP expressed in the nuclei (arrow) and the membrane (arrowhead) of D. melanogaster wild-type Berlin (wtB) spermatheca secretory cells (G), and absent from D. sechellia (14021–0248.25) (H) and D. melanogaster DGRP-357 (DGRP-357) (I). Scale bar 20 μm.

DOI: http://dx.doi.org/10.7554/eLife.03785.018

Figure 5—figure supplement 1. Egg hatching in morinda.

(A) D. melanogaster DGRP-357 first-instar larvae (L1) hatching inside the female reproductive system. U: uterus; S: spermatheca; SR: seminal receptacle. Scale bar 20 μm. (B) Proportion of eggs hatched or moulted to larva 1 (L1) larva 2 (L2) or larva 3 (L3), in D. melanogaster wild-type Berlin (wtB), D. melanogaster pferta (pferta), D. melanogaster Oregon-R (OR), D. melanogaster Canton-S (CS), D. melanogaster DGRP-357 (DGRE-357) D. melanogaster DGRP-437 (DGRP-437), D. simulans (14021–0251.004, sim) and D. mauritiana (14021–0241.01, mau).

DOI: http://dx.doi.org/10.7554/eLife.03785.019