FIG. 1.
Angiotensin II infusion increased CCN2 expression and O2•− production in the vascular wall. (A) Representative CCN2 inmunostaining and fluorescent confocal photomicrographs of O2•−, measured as DHE staining in aortic serial sections from saline and AngII-infused mice after 7 days. (B) Gene expression of CCN2 was evaluated by real-time PCR. Data are expressed as mean±SEM of fold increase saline-infused control of five to six animals per group. *p<0.05 versus saline-infused mice. AngII, angiotensin II; DHE, dihydroethidium; O2•−, superoxide anion; PCR, polymerase chain reaction. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars