Reagent	Type	Manufacturer	Catalog #	Comments
pLEX-TRC206 SK-MEL-5	Cells	Original authors	n/a	Engineered to express GFP
Synergy HT Microplate Reader*	Equipment	Bio-Tek		Original equipment used: Molecular Devices SpectraMax M5e Microplate Reader
384-well clear-bottomed plates	Material	Corning	3712
Phenol red free DMEM*	Medium	Sigma-Aldrich	D1145	Original unspecified. This formulation of DMEM does not contain L-glutamine or sodium pyruvate, so these will be supplemented to the medium.
Sodium pyruvate solution*	Reagent	Sigma-Aldrich	S8636
FBS*	Reagent	Sigma-Aldrich	F4135	Original unspecified
100X Pen–Strep–Glut*	Reagent	Sigma-Aldrich	G1146	Original from Invitrogen (15,140-122)
Puromycin dihydrochloride	Reagent	Sigma-Aldrich	P9620	Original unspecified
Biomek FX	Equipment	Beckman Coulter		Communicated by authors. Original from Thermo Scientific (Combi reagent dispenser)