Reagent	Type	Manufacturer	Catalog #	Comments
pLEX-TRC206 SK-MEL-5	Cells	Original authors	n/a	Engineered to express GFP
PLX4720	Drug	Chemietek	CT-P4720
PD184352	Drug	Santa Cruz	sc-202759A	MEK inhibitor
384-well clear-bottomed plates	Material	Corning	3712
0.45 µm syringe filter	Materials	Sigma-Aldrich	Z355518	Original unspecified
10 ml syringe	Materials	Sigma-Aldrich	Z116874	Original unspecified
Phenol red free DMEM*	Medium	Sigma-Aldrich	D1145	Original unspecified. This formulation of DMEM does not contain L-glutamine or sodium pyruvate, so these will be supplemented to the medium.
Sodium pyruvate solution*	Reagent	Sigma-Aldrich	S8636
FBS*	Reagent	Sigma-Aldrich	F4135	Original unspecified
100X Pen–Strep–Glut*	Reagent	Sigma-Aldrich	G1146	Original from Invitrogen (15,140-122)
HGF	Reagent	Sigma-Aldrich	H5791	Original from RayBiotech (228-10,702-2)
DMSO*	Reagent	Sigma-Aldrich	D8418	Original unspecified
Synergy HT Microplate Reader*	Equipment	Bio-Tek		Original equipment used: Molecular Devices SpectraMax M5e Microplate Reader
Biomek FX	Equipment	Beckman Coulter		Communicated by authors. Original from Thermo Scientific (Combi reagent dispenser) and CyBio robotic liquid handler.