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. 2015 Jan 1;22(1):48–62. doi: 10.1089/ars.2013.5803

FIG. 2.

FIG. 2.

The absence of MSRA-1 exacerbates synaptic dysfunction in the Aβ transgenic worms. (A) The graph shows the time course of paralysis in response to nicotine (31 mM). One-day-old control worms (CL2122), Aβ transgenic worms (CL2120), Aβ; msra-1 worms (ANM19), and Aβ; msra-1 worms rescued with the wild-type msra-1 gene (ANM45) were assayed on nicotine plates. The absence of MSRA-1 increases the nicotine resistance of the Aβ strain. Aβ; msra-1 rescued strain ANM45 shows the same behavior as the Aβ strain. Data are means±SEM from at least four independent experiments. n≥45 animals per strain in each experiment. Repeated-measures two-way ANOVA followed by Bonferroni's post hoc comparisons tests were used for statistical analysis. ***p≤0.001 comparing the Aβ transgenic worms with Aβ; msra-1 worms. ++p≤0.01 comparing Aβ transgenic worms with the control worms. +++p≤0.001 comparing Aβ transgenic worms with the control worms. (B) One-day-old control worms (CL2122), Aβ transgenic worms (CL2120), and Aβ; msra-1 animals (ANM19) were assayed on levamisole plates (0.25 mM). Data are means±SEM from at least four independent experiments. n≥45 animals per strain in each experiment. The paralysis curves show no differences between the strains. (C) The graph compares the paralysis of the Aβ expressing worms in different backgrounds (msra-1 or sod-3) after a 45-min exposure to nicotine 31 mM. The absence of both msra-1 and sod-3 worsens the nicotine resistance of the Aβ worms. Contrary to what we observe in the msra-1 mutant, the sod-3 mutants show nicotine resistance even in the absence of Aβ. Data are means±SEM from at least four independent experiments. n≥45 animals per strain in each experiment. One-way ANOVA followed by Bonferroni's post hoc comparisons tests were used for statistical analysis. ***p≤0.001 (D) The graph shows the time course of paralysis in response to aldicarb (1 mM). One-day-old control worms (CL2122), Aβ transgenic worms (CL2120), Aβ; msra-1 animals (ANM19), and msra-1 mutant worms were assayed on aldicarb plates. The absence of MSRA-1 does not change the hypersensitivity of the Aβ worms to aldicarb. Data are means±SEM from at least four independent experiments, n≥45 animals per strain in each experiment. Repeated-measures two-way ANOVA followed by Bonferroni's post hoc comparisons tests were used for statistical analysis. **p≤0.01, ***p≤0.001 comparing Aβ transgenic worms with the control worms.