FIG. 3.

MSRA-1 knockdown enhances the mislocalization of the nAChR ACR-16 in the NMJ of Aβ transgenic worms. (A) Control worms expressing ACR-16::GFP show fluorescent puncta over the ventral nerve cord (a). msra-1 knockdown does not alter the localization of ACR-16::GFP in muscle cells (b). Worms that express Aβ show a markedly altered localization of ACR-16::GFP puncta which can be seen in several muscle arms (arrows) (c). In the presence of Aβ, msra-1 knockdown significantly reduces ACR-16 fluorescence (d). (B) The graph shows the quantification of ACR-16::GFP fluorescence as integrated density of the puncta. Data are means±SEM from n≥30 worms per strain. Student's t-test was used for statistical analysis. ***p≤0.001. (C) Western blot of total protein extracts from strains shown in (A). ACR-16::GFP detected with anti-GFP antibodies. Anti-α-tubulin antibodies were used as a loading control. (D) The graph shows quantification of the Western blot in (C). Data are means±SEM of three independent Western blots. Student's t-test was used for statistical analysis.