Table 1.
Identification of Cysteine-Disulfide-Dependent Binding Partners of FOXO3
| Protein names | Gene names | Uniprot | Unique peptide | PEP | p-value | Fold change | MW (kDa) | Shift |
|---|---|---|---|---|---|---|---|---|
| FOXO3-Cys31 | ||||||||
| Peroxiredoxin-1 | PRDX1 | Q06830 | 10 | 3.4979E-38 | 0.016366613 | 19.41407143 | 22.11 | Yes |
| Peroxiredoxin-2 | PRDX2 | P32119 | 6 | 1.9653E-22 | 0.011488354 | 11.1392564 | 21.892 | Yes |
| Proteasome subunit beta type-6 | PSMB6 | P28072 | 3 | 2.4438E-09 | 0.015472578 | 5.407060481 | 25.357 | Yes |
| Calmodulin | CALM2;CALM1 | P62158 | 4 | 1.5769E-56 | 0.033803563 | 5.198066127 | 20.762 | N.I. |
| Importin-7 | IPO7 | O95373 | 19 | 3.2462E-222 | 0.009029563 | 4.912392441 | 119.52 | Yes |
| Peroxiredoxin-5 | PRDX5 | P30044 | 7 | 5.636E-23 | 0.004519624 | 4.778972665 | 22.086 | N.D. |
| FOXO3-Cys150 | ||||||||
| Importin-7 | IPO7 | O95373 | 19 | 3.2462E-222 | 0.000259916 | 37.96989779 | 119.52 | Yes |
| Importin-8 | IPO8 | O15397 | 15 | 6.9487E-154 | 0.000508687 | 19.41086975 | 119.94 | Yes |
| Peroxiredoxin-1 | PRDX1 | Q06830 | 10 | 3.4979E-38 | 0.037154983 | 11.45135205 | 22.11 | Yes |
| Transportin-1 | TNPO1 | Q92973 | 9 | 4.0284E-43 | 0.005145949 | 8.134666145 | 102.35 | Yes |
| Ubiquitin-like modifier-activating enzyme 1 | UBA1 | P22314 | 11 | 3.4263E-99 | 0.004634444 | 7.181045658 | 117.85 | N.D. |
| Proteasome subunit beta type-6 | PSMB6 | P28072 | 3 | 2.4438E-09 | 0.01122133 | 5.314328148 | 25.357 | Yes |
| Peroxiredoxin-2 | PRDX2 | P32119 | 6 | 1.9653E-22 | 0.045877289 | 4.954137135 | 21.892 | Yes |
| Importin subunit beta-1 | KPNB1 | Q14974 | 5 | 3.6065E-25 | 0.002571971 | 4.249026831 | 97.169 | N.D. |
| FOXO3-Cys190 | ||||||||
| Glutathione S-transferase omega-1 | GSTO1 | P78417 | 9 | 2.3023E-28 | 0.006422468 | 9.394772677 | 27.566 | N.I. |
| Proteasome subunit alpha type-4 | PSMA4 | P25789 | 6 | 1.3338E-16 | 0.008221979 | 6.961045517 | 29.483 | N.D. |
| Proteasome subunit beta type-6 | PSMB6 | P28072 | 3 | 2.4438E-09 | 0.025890331 | 5.380638158 | 25.357 | Yes |
| Arginase-1 | ARG1 | P05089-2 | 4 | 5.8174E-08 | 0.003837175 | 4.647008858 | 35.664 | Yes |
| FOXO3-Cys362 | ||||||||
| Peroxiredoxin-2 | PRDX2 | P32119 | 6 | 1.9653E-22 | 0.043097546 | 5.60347922 | 21.892 | Yes |
| Proteasome subunit alpha type-4 | PSMA4 | P25789 | 6 | 1.3338E-16 | 0.031961578 | 4.377192513 | 29.483 | N.D. |
| Peptidyl-prolyl cis-trans isomerase A | PPIA | P62937 | 3 | 8.4601E-09 | 0.03539262 | 4.111145097 | 18.012 | N.D. |
| FOXO3-Cys622 | ||||||||
| Cyclin-dependent kinase 4 | CDK4 | P11802 | 6 | 5.4088E-22 | 1.54875E-05 | 10.81388506 | 33.729 | Yes |
| Peroxiredoxin-1 | PRDX1 | Q06830 | 10 | 3.4979E-38 | 0.039055561 | 9.240073572 | 22.11 | Yes |
| Peroxiredoxin-5 | PRDX5 | P30044 | 7 | 5.636E-23 | 7.90351E-05 | 8.809030507 | 22.086 | N.D. |
| Peroxiredoxin-2 | PRDX2 | P32119 | 6 | 1.9653E-22 | 0.001943848 | 7.126900789 | 21.892 | Yes |
| Proteasome subunit alpha type-4 | PSMA4 | P25789 | 6 | 1.3338E-16 | 0.015236304 | 6.8761906 | 29.483 | N.D. |
| Glyceraldehyde-3-phosphate dehydrogenase | GAPDH | P04406 | 14 | 1.1354E-191 | 0.001033415 | 5.413238551 | 36.053 | Yes |
| Glutathione S-transferase omega-1 | GSTO1 | P78417 | 9 | 2.3023E-28 | 0.034872177 | 4.706508784 | 27.566 | N.I. |
| Reticulocalbin-2 | RCN2 | F8WCY5 | 6 | 1.3415E-96 | 0.032031865 | 4.441045678 | 39.139 | N.I. |
N.I., not identified in secondary screen; N.D., mass-shift not observed in secondary screen.