Figure 4.
(A–D) hBMMSCs demonstrated pluripotent differentiation potential in vitro and in vivo. (A) In vivo osteogenesis test: Bone tissue formed subcutaneously on the back of the nude mice 35 days after hBMMSCs inoculation. (B) Histology results showed endochondral bone formation (hematoxylin and eosin staining, ×200) (C) Histology results showed simultaneous bone tissue formation (hematoxylin and eosin staining, ×100) (D) In vitro adipogenesis test: Sudan black B staining lipid droplets appeared at day 18 post 113 induction of adipogenesis. hBMMSCs, human bone marrow mesenchymal stem cells.