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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Pathog Dis. 2014 May 16;71(3):336–351. doi: 10.1111/2049-632X.12179

Figure 6. CPAF cleaves LAP1 during infection.

Figure 6

A.) CPAF cleaves EGFP-LAP1. HeLa lysates transfected (+) or not (-) with EGFP-LAP1 were incubated with (r) or without (-) recombinant CPAF or with lysates from L2-infected HeLa cells (L2) or mock-infected (m) HeLa cells. Blots were probed with anti-EGFP (EGFP) or anti-LAP1 (LAP1). B.) LAP1 cleavage during infection is CPAF-dependent. HeLa cells infected with either the CPAF-strain or RifR (WT) strain or mock-infected were harvested in 1% SDS buffer at the indicated times post-infection. Blots were probed with anti-LAP1 and anti-rpoB. Arrows indicate LAP1 cleavage products.